人脂联素蛋白
种类: 重组蛋白 货号: 41013
Tag: Flag 型号: 0.1 mg
来源: HEK 293 纯度: >98%
别名: Acrp30, GBP28, AdipoQ 种类: Human

 

Introduction to the Molecule

Adiponectin is an adipokine exclusively expressed in adipose tissues with potent anti-diabetic, anti-atherogenic and anti-inflammatory functions.


In humans, decreased serum adiponectin levels are associated with increased body mass index (BMI), decreased insulin sensitivity, less favourable plasma lipid profiles, increased inflammation and increased risk for the development of type 2 diabetes, hypertension and coronary heart diseases.

 

Description

Total 234 AA. Mw: 25.5kDa (calculated). C-terminal flag-tag, 8 extra AA(highlighted).

 

Amino Acid Sequence

ETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGV

PGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNI

PGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGE

RNGLYADNDNDSTFTGFLLYHDTNDYKDDDDK

 

Formulation

Lyophilized in 1 mg/mL in PBS.

 

Endotoxin Level

<0.2 EU/ug.

 

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let

the lyophilized pellet dissolve completely.

 

Storage

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.

Avoid repeated freezing /thawing cycles.

 

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

 

Applications

Cell culture, ELISA and Western blotting.

 

SDS-PAGE Gel