人成纤维因子21(FGF-21)
种类: 重组蛋白 货号: 41184
Tag: His 型号: 0.1 mg
来源: E.Coli 纯度: >95%
别名: FGF21 种类: Human

 

Introduction to the Molecule

FGF21, a polypeptide with 210 amino acid residues produced mostly from the liver tissue. Mouse FGF21 shares 75% identity as human FGF21. Recent animal studies indicate it possesses potent beneficial effects on glucose and lipid metabolism and insulin sensitivity. Increasing data shows FGF21 can significantly stimulate glucose uptake in mature adipocytes. And The lowered LDL-cholesterol and increased HDL-cholesterol can also be observed. FGF21 exerts its bioactivity through interaction with membrane-bound FGF receptors (FGFRs) which requires β-Klotho as a co-factor to bind and activate FGFR signaling. The activation of FGF21 can induce the stimulation of diverse downstream pathways mediated by MAPK,FRS-2, SHP-2, PI3K, raf, stat and other signaling molecules. In sum, FGF21 induces a variety of significant beneficial metabolic changes without apparent adverse effects which makes this factor a hot candidate to treat some metabolic diseases.

 

Description

Total 206AA Mw: 22.5kDa (calculated). N-terminal His-tag and TEV cleavage site, 25extra AA (highlighted).

 

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAHPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDG

TVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVY

QSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGPSQ

GRSPSYAS

 

Formulation

Lyophilized in 1 mg/mL in PBS.

 

Endotoxin Level

<0.2 EU/ug.

 

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.

 

Storage

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.

Avoid repeated freezing /thawing cycles.

 

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

 

Applications

ELISA and Western blotting.

 

SDS-PAGE Gel