FGF21 is a metabolic hormone mainly produced in liver. It regulates glucose and lipid metabolism through pleiotropic actions in multiple tissues, and protects against metabolic damages under various stresses.
In humans, high circulating FGF21 levels are found in obesity and multiple cardiometabolic disorders, including metabolic syndrome, type 2 diabetes, non-alcoholic fatty liver disease and coronary artery disease. Serum FGF21 is a potential biomarker for the early detection and risk prediction of these diseases.
TYPICAL STANDARD CURVE
The following standard curve is provided for demonstration only. A standard curve should be generated for each assay.
Human FGF-21 standard curve (4-parameter)
The lowest level of human FGF-21 that can be detected by this assay is 15 pg/ml.
Cross Reactivity of recombinant proteins
Intra-assay Precision (Precision within an assay)
Two samples of known concentration were tested 12 times on one plate.
Inter-assay Precision (Precision between assays)
Two samples of known concentration were tested in 10 separate assays.
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FGF-21 were serially diluted with the 1×Assay buffer to produce samples with values within the dynamic range of the assay.